317640-M

Dipeptidyl Peptidase IV (CD26), Porcine Kidney

• Synonim(y): CD26, DPPIV
• UNSPSC Code: 12352202
• Numer WE: 3.4.14.5 (BRENDA, IUBMB)
• NACRES: NA.54
• Specific activity: ≥35 units/mg protein
• Assay: ≥90% (SDS-PAGE)

Właściwości

• assay: ≥90% (SDS-PAGE)
• form: liquid
• specific activity: ≥35 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• storage temp.: −70°C

Opis

General description

Native, DPP IV purified from porcine kidney (renal brush border of the proximal tubule). A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. Cleaves Xaa-Pro dipeptides from the N-terminus of oligo and polypeptides. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, T cell activation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. MW: ~210000-260000 (unreduced).

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.

Preparation Note

Dilute only the amount of enzyme needed for each assay. Upon thawing, do not re-freeze, store in the refrigerator (4°C).

Other Notes

Bar, J., et al. 2003. Biol. Chem.384, 1553.
Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA100, 5063.
Ikehara, Y., et al. 1994. Methods. Enzymol.244, 215.
David, F., et al. 1993. J. Biol. Chem.268, 17247.
Thomsen, P.D., et al. 1993. Mamm. Genome4, 604.
Misumi, Y., et al. 1992. Biochim. Biophys. Acta.1131, 333.
Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler372, 213.
Checler, F., et al. 1985. J. Neurochem.45, 1509.
Imai, K., et al. 1983. J. Biochem.93, 431.
Kato, T., et al. 1979. Experientia.35, 409.
Kojima, K., et al. 1979. Anal. Biochem.100, 43.
Kato, T., et al. 1978. Biochem. Med.19, 351.
Kenny, A.J., et al. 1976. Biochem. J.157, 169.

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmole 7-(Gly-Pro)-amino-4-methylcoumarinamide per min at 37°C, pH 8.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• Co się dzieje?: WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable