17-294

Zestaw do testu aktywacji Rho

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

• UNSPSC Code: 12161503
• NACRES: NA.84
• eCl@ss: 32161000

Właściwości

• Quality Segment: 100
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable, affinity binding assay: suitable
• NCBI accession no.: NM_001664.2
• UniProt accession no.: P08134, P61586, P62745
• detection method: chemiluminescent, colorimetric, fluorometric
• shipped in: dry ice
• Gene Information: human ... RHOA(387)

Opis

General description

A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.

Application

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

Packaging

Kit capacity: 30 assays

Analysis Note

Routinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).

Other Notes

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)

Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS05,GHS09
• signalword: Danger
• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 3
• hcodes: H318,H410,EUH430
• pcodes: P201 - P202 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1