Przejdź do zawartości
Merck

17-294

Zestaw do testu aktywacji Rho

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

Slide 1 of 1
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.

Wybierz wielkość

17-294

4430,00 zł

4430,00 zł

Przewidywany termin wysyłki16 marca 2026Szczegóły

​

Informacje o tej pozycji

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

Kluczowe dokumenty

Wyświetl całą dokumentację

Przejdź do

Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomóc

Quality Level

100

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable, affinity binding assay: suitable

NCBI accession no.

NM_001664.2

UniProt accession no.

P08134, P61586, P62745

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

dry ice

Gene Information

human ... RHOA(387)

Porównaj podobne pozycje

Wyświetl pełne porównanie

Pokaż różnice

1 of 4

Ta pozycja
14-38314-45514-278
Rho Activation Assay Kit PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

Sigma-Aldrich

17-294

Rho Activation Assay Kit

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

Sigma-Aldrich

14-383

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg Reagent (Ral GDS-RBD, agarose). A GST-tagged fusion protein, corresponding to amino acids 788-884 of human Ral GDS-Rap binding domain (RBD), for use in Affinity Binding Assays.

Sigma-Aldrich

14-455

Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg

Ras Assay Reagent (Raf-1 RBD, agarose)

Sigma-Aldrich

14-278

Ras Assay Reagent (Raf-1 RBD, agarose)

detection method

chemiluminescent, fluorometric, colorimetric

detection method

-

detection method

-

detection method

-

Gene Information

human ... RHOA(387)

Gene Information

mouse ... RTKN(20166)

Gene Information

human ... TERF2IP(54386)

Gene Information

human ... MRAS(22808)

technique(s)

activity assay: suitable, affinity binding assay: suitable

technique(s)

affinity binding assay: suitable

technique(s)

affinity binding assay: suitable

technique(s)

activity assay: suitable

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

NCBI accession no.

NM_001664.2

NCBI accession no.

NM_001015055.1, NM_001015056.1, NM_033046.2

NCBI accession no.

NM_018975.2

NCBI accession no.

NM_001085049.1, NM_012219.3

General description

A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.

Application

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

Packaging

Kit capacity: 30 assays

Analysis Note

Routinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).

Other Notes

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)

Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ta strona może zawierać tekst przetłumaczony maszynowo.

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Klasa składowania

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

M P Gratacap et al.
The Journal of biological chemistry, 276(51), 47906-47913 (2001-09-19)
Platelets were used to study the activation of Rho and Rac through G-protein-coupled receptors and its regulation by cyclic nucleotides. The thromboxane A(2) (TXA(2)) mimetic rapidly activated both small GTPases independently of integrin alpha(IIb)beta(3) activation., which leads to the activation
Toshifumi Sugatani et al.
The Journal of biological chemistry, 278(7), 5001-5008 (2002-12-04)
PTEN (also known as MMAC-1 or TEP-1) is a frequently mutated tumor suppressor gene in human cancer. PTEN functions have been identified in the regulation of cell survival, growth, adhesion, migration, and invasiveness. Here, we characterize the diverse signaling networks
Jeanne M Manganello et al.
The Journal of biological chemistry, 278(1), 124-130 (2002-10-26)
The present studies mapped the protein kinase A (PKA) phosphorylation site of Galpha(13) and studied the consequences of its phosphorylation. Initial experiments using purified human Galpha(13) and the PKA catalytic subunit established that PKA directly phosphorylates Galpha(13). The location of
Geraldine Pawlak et al.
The Journal of biological chemistry, 277(30), 26927-26933 (2002-05-16)
Cellular transformation by v-Src is believed to be caused by aberrant activation of signaling pathways that are normally regulated by cellular Src. Using normal rat kidney cells expressing a temperature-sensitive mutant of v-Src, we examined the role of the Raf/MEK/ERK
Y Yamaguchi et al.
The Journal of biological chemistry, 276(22), 18977-18983 (2001-03-30)
The Rho family of small GTPases has been shown to be involved in the regulation of neuronal morphology, and Rac and Rho exert antagonistic actions in neurite formation. In this study, we have examined the cross-talk between Rac and Rho
Wyświetl wszystkie powiązane dokumenty

Powiązane treści

Hallmarks of Aging

"Aging: getting older, exhibiting the signs of age, the decline in the physical (and mental) well-being over time, leading to death. Since the beginning of time, man has been obsessed with trying to slow down, stop, or even reverse the signs of aging. Many have gone as far as experimenting with nutritional regimens, eccentric exercises, fantastic rituals, and naturally occurring or synthetic wonder-elements to evade the signs of normal aging. Biologically speaking, what is aging? And what does the latest research tell us about the possibility of discovering the elusive “fountain of youth”? Many advances in our understanding of aging have come from systematic scientific research, and perhaps it holds the key to immortality. Scientifically, aging can be defined as a systems-wide decline in organismal function that occurs over time. This decline occurs as a result of numerous events in the organism, and these events can be classified into nine “hallmarks” of aging, as proposed by López-Otin et al. (2013). Several of the pathologies associated with aging are a direct result of these events going to extremes and may also involve aberrant activation of proliferation signals or hyperactivity. The hallmarks of aging have been defined based on their fulfillment of specific aging related criteria, such as manifestation during normal aging, acceleration of aging if experimentally induced or aggravated, and retardation of aging if prevented or blocked, resulting in increased lifespan. The nine hallmarks of aging are genomic instability, telomere attrition, epigenetic alterations, loss of proteostasis, deregulated nutrient sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, and altered intercellular communication. The biological processes underlying aging are complex. By understanding the hallmarks in greater detail, we can get closer to developing intervention strategies that can make the aging process less of a decline, and more of a recline."

Questions

Reviews

No rating value

Active Filters

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej