171272

Aktywator AMPK VI, RSVA314

The AMPK Activator VI, RSVA314 modulates the biological activity of AMPK. This small molecule/inhibitor is primarily used for Biochemicals applications.

• Synonim(y): RSVA314, aktywator sygnalizacji AMPK VI
• Wzór empiryczny (zapis Hilla): C₁₈H₂₁N₃O₃
• Numer CAS: 316130-82-2
• Masa cząsteczkowa: 327.38
• MDL number: MFCD00008216
• NACRES: NA.51
• UNSPSC Code: 12352200
• Assay: >90% (HPLC)
• Form: solid
• Storage condition: OK to freeze, protect from light

Właściwości

• Quality Segment: 100
• assay: >90% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: yellow
• solubility: DMSO: 50 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Opis

General description

A cell permeable hydrozide that activates AMPK indirectly via a CaMKKβ-dependent pathway similar to resveratrol (Cat. No. 554325), which results in the phosphorylation of Thr-172 on the AMPKα2 subunit. This activator promotes lysosomal amyloid-β degradation in 3 cell lines nearly 40 times more potently than resveratrol with an IC₅₀ of 1-2 µM. Treatment with RSVA314 at 1-3 µM results in a dose-dependent inhibition of the phosphorylation of 3 proteins downstream from mTOR activation including p70S6K, eukaryotic elongation factor 2 kinase (eEF2K), and S6 ribosomal protein, and promotes a robust increase in the phosphorylation of the protein raptor at Ser-792 in western blots. At the same concentrations, it elicits a strong, dose-dependent conversion of the light chain 3 (LC3) protein, a marker involved with autophagy induction, from LC3-I and LC3-II, also in western blots.

Packaging

Packaged under inert gas

Other Notes

Vingtdeux, V., et al. 2011. FASEB J.25, 219.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable