1.50442

SeQuant^® ZIC^®-HILIC 100Å (3.5 µm) HPLC Columns

L × I.D. 150 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

• Synonim(y): Zwitterionowa kolumna do chromatografii cieczowej HPLC o oddziaływaniu hydrofilowym
• UNSPSC Code: 41115709
• NACRES: NB.21
• Particle size: 3.5 μm
• Matrix active group: ZIC-HILIC, ZIC-HILIC bonding phase
• Pore size: 100 Å pore size
• Matrix: fully porous particle

Właściwości

• Nazwa produktu: SeQuant^® ZIC-HILIC^™ 3.5µm,100Å 150 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
• material: PEEK-lined stainless steel column
• Quality Segment: 100
• agency: suitable for USP L114
• product line: SeQuant^®
• feature: endcapped: no
• parameter: 550 bar pressure, 70 °C max. temp.
• technique(s): HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)
• column L × I.D.: 15 cm × 2.1 mm
• matrix: fully porous particle
• matrix active group: ZIC-HILIC, ZIC-HILIC bonding phase
• particle size: 3.5 μm
• pore size: 100 Å pore size
• operating pH: 2-7.5
• separation technique: hydrophilic interaction (HILIC)
• storage temp.: no temp limit

Opis

General description

SeQuant^® ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.The column dimension of 150 mm length x 2.1 mm ID is the optimal choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant^® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^® ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Application

• Synteza słabych kationowymiennych/C(18) bifunkcyjnych polimerów magnetycznych: Wykazano ich zastosowanie w obróbce wstępnej i oznaczaniu glufosynatu i jego metabolitów w osoczu, zwiększając możliwości zastosowań bioanalitycznych (Sun D, Xu Z, 2024).
• Wysoce czuły pomiar niedowartościowanego fosfoenolopirogronianu: Wykorzystuje EDTA-facilitated HILIC-MS do dokładnego wykrywania w próbkach biologicznych, co ma kluczowe znaczenie dla badań metabolicznych (Shen D, Wang N, 2024).
• Porównanie kolumn RP, HILIC i PGC dla toksykometabolomiki: Ocena różnych technik chromatograficznych do kompleksowego profilowania metabolicznego w biomedycynie (Hemmer S, Meyer MR, 2024).

Analysis Note

Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.40

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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