14-197-M

Białko kinazy kazeinowej 2, aktywne, 10 µg

Active, recombinant full-length human Casein Kinase 2 protein, containing an α-subunit N-terminal 6His tag & a β-subunit N-terminal GST tag. For use in Kinase Assays.

• UNSPSC Code: 12352202
• NACRES: NA.41
• eCl@ss: 32160405
• Biological source: human
• Recombinant: expressed in Sf21 cells

Właściwości

• biological source: human
• Quality Segment: 100
• recombinant: expressed in Sf21 cells
• mol wt: α-subunit M_w 49 kDa (heterotetramer; α₂β₂), β subunit M_w 53 kDa (heterotetramer; α₂β₂)
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_001895.3, NM_177559.2, NM_177560.2
• UniProt accession no.: P68400

Opis

General description

Product Source: Expressed in Sf21 insect cells.

Recombinant full-length human Casein Kinase 2 protein, containing an &alpyha;-subunit N-terminal 6His tag and a β-subunit N-terminal GST tag.

Biochem/physiol Actions

Protein Target: CK2

Packaging

2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size.

Physical form

20mM MOPS, pH 7.4, 1mM EDTA, 0.1% (v/v) Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol.

Sequential chromatography using heparin Sepharose^™ and Mono Q anion exchange resin

Preparation Note

1 year at -20°C.

Analysis Note

Routinely evaluated by phosphorylation of the Casein Kinase 2 Substrate Peptide.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

Sepharose is a trademark of Cytiva

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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