806595

APN-Azide

95%

• Synonim(y): 3-(4-Azidophenyl)propiolonitrile, APN-N₃
• Wzór empiryczny (zapis Hilla): C₉H₄N₄
• Numer CAS: 1643841-88-6
• Masa cząsteczkowa: 168.15
• MDL number: MFCD29059836
• UNSPSC Code: 12352125
• PubChem Substance ID: 329769021
• NACRES: NA.22

Właściwości

• Quality Segment: 100
• assay: 95%
• form: powder
• reaction suitability: reagent type: cross-linking reagent
• mp: 100 °C
• functional group: azide
• storage temp.: 2-8°C
• SMILES string: N#CC#CC1=CC=C(N=[N+]=[N-])C=C1
• InChI: 1S/C9H4N4/c10-7-1-2-8-3-5-9(6-4-8)12-13-11/h3-6H
• InChI key: BDLXITYEYYHQNK-UHFFFAOYSA-N

Opis

Application

APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.

Standard protein labeling procedure (cysteine labeling)
1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
3. Incubate at room temperature for 2 hours.
4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.
5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.
*Note: avoid thiol-containing buffers.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02,GHS07
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H228,H315,H319,H335
• Ostrzeżenia: P210 - P240 - P241 - P261 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2 - STOT SE 3
• Narządy docelowe: Respiratory system
• Klasa składowania: 4.1B - Flammable solid hazardous materials
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable