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V900843

Sigma-Aldrich

Tris-Borate-EDTA buffer

Vetec™, reagent grade, 10 ×, solution, RNase and DNase free

Synonym(s):

TBE buffer

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About This Item

MDL number:
MFCD00236358
UNSPSC Code:
41105319
PubChem Substance ID:
329830197

grade

reagent grade

product line

Vetec™

form

solution

feature

RNase and DNase free

concentration

10 ×

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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Application

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H360FD

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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