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G1007628

HPLC Analysis of Benalaxyl Enantiomers on Astec CYCLOBOND I 2000 HP-RSP in Reversed Phase Mode

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Astec CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)
mobile phase: [A] 10 mM ammonium acetate, pH 4.0 with acetic acid; [B] acetonitrile; (70:30, A:B)
flow rate: 1 mL/min
pressure: 1550 psi (107 bar)
column temp.: 25 °C
detector: UV, 220 nm
injection: 10 μL
sample: 250 μg/mL in 75:25, water:methanol

suitability

application for HPLC

application(s)

agriculture
environmental

Analysis Note

Benalaxyl is a fungicide used to protect a variety of crops, including potatoes and grapes. It is a chiral compound, comprising two enantiomers. Enantioselective influences of chiral pesticides, like Benalaxyl, and their metabolites on ecosystems is an area of great importance. The possibility that only one enantiomer has the wanted properties and/or the other form an unwanted effect may exist. An analytical separation of the enantiomers is required to perform studies in these areas. The Astec CYCLOBOND I 2000 HP-RSP HPLC column resolves the enantiomers of Benalaxyl under reversed-phase and LC/MS-compatible conditions.

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Analyte

Description
    Benalaxyl, PESTANAL®, analytical standard

analytical column

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