TP0100

Total Protein Kit, Micro

• Synonym(s): Bradford
• NACRES: NA.32
• UNSPSC Code: 12352202

Properties

• description: wavelength 595 nm; total test time 2 min; working reagent stability 18-26°C for 2 weeks; dynamic range 50 mg/dL 0.5 g/L
• parameter: 18-26 °C temperature, 50 μL sample volume
• storage temp.: 2-8°C
• Quality Level: 100

Description

General description

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Application

Colorimetric, Endpoint

Suitable for protein determination for samples of up to 0.5 mg/ml.

Total protein kit, micro has been used to determine:

• total protein in the bacterial homogenate by Bradford method[1]
• total protein content by the Bradford microassay from the tissue extracts[2]
• serum total protein[3]
• soluble protein content by the Bradford method from leaves[4]

Biochem/physiol Actions

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.

Total protein kit, micro can be used to determine the concentration of proteins in solution.

Kit Components Also Available Separately

• Protein Standard 5 mL

Safety Information

• pictograms: GHS02,GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H226,H290,H302 + H312 + H332,H314,H317,H370,H411
• pcodes: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1
• target_organs: Eyes,Central nervous system
• Storage Class: 3 - Flammable liquids
• flash_point_f: 132.8 °F
• flash_point_c: 56 °C
• wgk: WGK 3