The molarity for T6455 is 200 mM Tris HCL, 0.5% ProClin, 10mM proprietary reagent, and approximately 96% water.
T6455
Tris-HCl Buffer, pH 10, 10×, Antigen Retriever
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Please contact Customer Service for Availability | PLN 467.00 |
1000 mL | Please contact Customer Service for Availability | PLN 1,010.00 |
About This Item
NACRES:
NA.47
UNSPSC Code:
12352107
Application
Tris-HCl Buffer, pH 10, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Tris-HCl buffer, pH 10, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.
Preparation Note
Dilute the Tris-HCl Buffer, pH 10, 10×, and Antigen Retriever 10-fold with water to prepare a 1 × Working Solution, e.g., dilute 10 mL of 10 × concentrate with 90 mL of water.
Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.
Other Notes
Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer. Store the prepared 1′ Tris-HCl Buffer, pH 10, Working Solution at 2–8 °C.
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This Item | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 500 |
| application(s) hematology | application(s) hematology | application(s) hematology | application(s) hematology |
| pH 10 | pH 6.0 | pH 8.5 | pH 6.3(concentrate) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| biological source synthetic | biological source - | biological source - | biological source - |
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the molarity of Tris-HCl Buffer, pH 10, 10× Antigen Retriever, catalog No T6455?
1 answer-
Helpful?
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What is the concentration of the product in terms of molarity
1 answer-
The concentration is as follows:
Tris HCL 200 mM
ProClin 0,5%
Proprietary reagent 10mM
Water ~96%Helpful?
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