T3069

Trizma^® hydrochloride solution

pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture

• Synonym(s): Tris hydrochloride solution
• NACRES: NA.25
• UNSPSC Code: 12161700

Properties

• Quality Segment: 200
• grade: BioPerformance Certified, Molecular Biology
• form: solution
• concentration: 2 M
• technique(s): cell culture | mammalian: suitable
• impurities: DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)
• color: colorless
• pH: 7.95—8.05, 8.0
• useful pH range: 7.0-9.0
• density: 1.07—1.09 g/cm³
• absorption: ≤0.05 at 290 at 40%

Description

General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Tris(hydroxymethyl)aminomethane (Tris) has a pH buffering range between 7.0 - 9.0 and is a routine buffer in biological sciences. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. It displays negligible binding towards metal in solutions.

Application

Trizma^® hydrochloride solution has been used as a component of lysis buffer for testing on commercially available biological sample preservation paper types. It has also been used as a vehicle and for dissolving leptin in reporter gene assay studies.[1]

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany