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T1032

TNM-FH insect medium

With L-glutamine, without sodium bicarbonate, powder, suitable for insect cell culture

Synonym(s):

Graces Modified Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00243216
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Quality Segment

500

form

powder

concentration

50.6 g/L

technique(s)

cell culture | insect: suitable

suitability

suitable for culture of other lepidopteran cell lines (Originally developed for the culture of Trichoplusia ni cells)

components

L-glutamine: 0.6 g/L
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

General description

A number of modifications of Grace′s medium were developed in several laboratories in the late 1960′s and 1970′s. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

  1. Good afternoon, I purchased TNM-FH insect medium (T1032-1L) and received it as a powder, not liquid. Could you provide instructions to prepare 1 L, including how much to weigh, total mass in the package, and if partial preparation is possible?

    1 answer

    1. This product formulation requires 50.6 grams to prepare 1L of medium. As powder medium is hygroscopic, it is best to prepare the entired contents of the 1L container at one time. This product also requires the addition of 350mg/L of sodium bicarbonate. General medium preparation instructions are provided at the link below:
      https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/powdered-media-preparation

      Please see the link below to review the poduct datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/112/696/t1032for.pdf

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