SRP5274

SIRT7, active, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): MGC126840, MGC126842, SIR2L7
• CAS Number: 1391453-59-0
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 112-139 RLU/min·ng
• mol wt: ~46 kDa
• NCBI accession no.: NM_016538
• application(s): genomic analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SIRT7(51547)

Description

General description

SIRT7 is a member of the class IV of sirtuin family of proteins which are homologs to the yeast Sir2 protein and play a role in cell differentiation, proliferation, apoptosis, metabolism, and senescence. SIRT7 associates with active rRNA genes and histones. Overexpression of SIRT7 increases pol I-mediated transcription whereas knockdown of SIRT7 or inhibition of its catalytic activity results in decreased association of pol I with rDNA and reduced pol I transcription. Depletion of SIRT7 stops cell proliferation and triggers apoptosis. SIRT7 deacetylates p53 and increases cellular resistance to cytotoxic and oxidative stress.

Physical form

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H360D
• Precautionary Statements: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable