SRP3252

SDF-1 alpha from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): CXCL12, PBSF, Stromal-Cell Derived Factor-1
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: rat
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 50.0-100.0 ng/mL
• mol wt: 7.9 kDa
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: Q9QZD1
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: rat ... CXCL12(24772)

Description

General description

Stromal cell-derived factor 1 α and β (SDF-1α and β) are stromal derived CXC chemokines, and signal through the C-X-C motif chemokine receptor-4 (CXCR4). SDF-1α is also known as C-X-C motif chemokine ligand 12 (CXCL12). Recombinant rat SDF-1α is a 7.9kDa protein containing 68 amino acid residues.

Application

Stromal cell-derived factor 1 α (SDF-1α ) has been used in cell migration assay and fibrin gel bead assay.

Biochem/physiol Actions

Stromal cell-derived factor 1 α and β (SDF-1α and β) chemoattract B and T cells, and have been shown to induce migration of CD34⁺ stem cells. Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor. SDF-1 α modulates leukocyte and hematopoietic precursor migration and pre-B cell proliferation.

Sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable