Skip to Content
Merck

Skip To

S6639

SSC Buffer 20× Concentrate

for Northern and Southern blotting, solution

SSC Buffer 20× Concentrate

Synonym(s):

20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
1 L
Please contact Customer Service for Availability
PLN 552.00

About This Item

NACRES:
NA.25
UNSPSC Code:
41105319

PLN 552.00


Please contact Customer Service for Availability

Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


grade

Molecular Biology

Quality Segment

description

Saline-Sodium Citrate buffer, made with ultrapure water

sterility

0.2 μm filtered

form

solution

color

colorless

pH

6.9 —7.1

application(s)

diagnostic assay manufacturing

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

General description

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Application

SSC Buffer 20× Concentrate has been used
  • as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence[1]
  • as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA[2]
  • as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Preparation Note

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
SRE0068S201593017
grade

Molecular Biology

grade

-

grade

Molecular Biology

grade

Molecular Biology

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

form

solution

form

liquid

form

solution

form

solution

pH

6.9 —7.1

pH

-

pH

-

pH

7.0±0.2

storage temp.

room temp

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp


Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

Reviews

Active Filters

  1. 1 Ratings-only review