S1629
Sulfatase from Aerobacter aerogenes
Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL
Synonym(s):
Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
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| Size/SKU | Availability | Price |
|---|---|---|
10 units | Please contact Customer Service for Availability | PLN 755.00 |
50 units | Please contact Customer Service for Availability | PLN 1,750.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
2-5 units/mg protein (biuret)
Concentration:
10-20 units/mL
PLN 755.00
Please contact Customer Service for Availability
type
Type VI
Quality Segment
form
buffered aqueous glycerol solution
specific activity
2-5 units/mg protein (biuret)
mol wt
~41 kDa
concentration
10-20 units/mL
foreign activity
β-Glucuronidase ≤10 U/mL
shipped in
wet ice
storage temp.
−20°C
General description
Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.[1]
Application
Sulfatase from Aerobacter aerogenes has been used:
- as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses[2]
- in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1[1]
- to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides[3]
Biochem/physiol Actions
Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging[4] and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis.[1] Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates.[5] Sulfatases find application in industry and agriculture.[1]
Physical form
Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity 2-5 units/mg protein (biuret) | specific activity - | specific activity - | specific activity 20-40 units/mg solid |
| concentration 10-20 units/mL | concentration ≥2,000 units/mL | concentration - | concentration - |
| form buffered aqueous glycerol solution | form aqueous solution | form powder | form lyophilized powder |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~41 kDa | mol wt ~85 kDa by gel filtration | mol wt ~85 kDa by gel filtration | mol wt ~43 kDa by gel filtration |
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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