P4439
Phosphatase, Alkaline from porcine kidney
lyophilized powder, ≥100 DEA units/mg protein
Synonym(s):
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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| Size/SKU | Availability | Price |
|---|---|---|
100 units | Please contact Customer Service for Availability | PLN 301.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
Specific activity:
≥100 DEA units/mg protein
PLN 301.00
Please contact Customer Service for Availability
form
lyophilized powder
Quality Segment
specific activity
≥100 DEA units/mg protein
composition
Protein, ≥50%
storage temp.
−20°C
Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling[1].
Biochem/physiol Actions
Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.
Packaging
Package size based on DEA units
Physical form
Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥100 DEA units/mg protein | specific activity ≥10 DEA units/mg solid | specific activity ≥5,700 DEA units/mg protein | specific activity ≥2,000 DEA units/mg protein |
| form lyophilized powder | form lyophilized powder | form (40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6) | form aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Protein, ≥50% | composition - | composition - | composition - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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