This product is not tested for solubility. However, other Protamine sulfate products currently available are dissolved initially in 6N Hydrochloric acid at 25 mg/mL. This stock solution may then be diluted out with aqueous buffer or medium. In addition, a brief search of the literature reveals that this material may also be prepared by dissolving in HPLC grade water to a concentration of 10mg/ml. A stock solution was diluted to a final working concentration in growth media of 10?g/ml and stored at -20°C. This information has not been validated.
Please see the link below to review this publication:
http://eprints.nottingham.ac.uk/11280/1/PhD_Thesis.pdf
P3369
Protamine sulfate
solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction
Synonym(s):
Salmine sulfate from salmon sperm, Protamine sulfate
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| Size/SKU | Availability | Price |
|---|---|---|
10 g | Please contact Customer Service for Availability | PLN 1,180.00 |
100 g | Please contact Customer Service for Availability | PLN 6,940.00 |
About This Item
Product Name
Protamine sulfate, meets USP testing specifications
agency
meets USP testing specifications
Quality Segment
assay
≥92% (chromatography), 90.0-110.0% dry basis
form
solid
mol wt
Mr 5000-10000
technique(s)
transduction: suitable
shipped in
ambient
storage temp.
2-8°C
General description
Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.
Application
Protamine sulfate has been used in the transduction of
- lentiviruses into human embryonic 293T cells[1]
- shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
- lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells
Biochem/physiol Actions
Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.
Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.
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This Item | |||
|---|---|---|---|
| assay ≥92% (chromatography), 90.0-110.0% dry basis | assay ≥98% (HPLC) | assay - | assay - |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 300 |
| form solid | form powder | form - | form solid |
| shipped in ambient | shipped in ambient | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. 15-25°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt Mr 5000-10000 | mol wt - | mol wt - | mol wt - |
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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In what solution can this particular protamine sulfate be dissolved? Sterile water, PBS, DMSO,...?
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