The total weight can be determined using the Unit Activity and the Protein Concentration reported on the lot specific Certificate of Analysis. For example, for the 1KU pack size, if the activity is 10 units/µl and the protein concentration is 0.384 mg/ml the weight would be 0.26mg.
P0996
PARP-1 human
recombinant, expressed in E. coli
Synonym(s):
NAD+ ADP-ribosyltransferase, Poly(ADP-ribose) Polymerase
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| Size/SKU | Availability | Price |
|---|---|---|
1000 units | Please contact Customer Service for Availability | PLN 3,410.00 |
About This Item
Recombinant:
expressed in E. coli
Quality Segment
recombinant
expressed in E. coli
form
solution
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... PARP1(142)
General description
Poly(ADP-ribose) polymerase 1 (PARP-1) is associated with the inflammation response during atherosclerosis. [1]
Poly(ADP-ribose) polymerase 1 (PARP1) belongs to the PARP family which is highly conserved during evolution. PARP1 gene is localized at 1q42.12 in the human chromosome.[2]
Application
PARP-1 human has been used in the GST-pull down assay for immunoprecipitation of Glutathione S-transferase (GST) or GST- cyclic GMP- AMP synthase (cGAS) (GST-cGAS). It has also been used in in vitro ribosylation assay.
PARP-1 is a nuclear enzyme that synthesizes ADP-ribose polymers from NAD+, specifically binds Zn2+ and DNA, and recognizes single-strand breaks in DNA. PARP1 has been used in a study to assess racial and tissue-specific cancer risk associated with polymorphism in the PARP1 gene. [3] It has also been used in a study to investigate inhibitors of PARP-1 for potential cancer treatments. [4]
Biochem/physiol Actions
PARP-1 is inactivated by cleavage into a 24kDA and 89kDA fragment by activated caspase-3 or caspase-7. This results in the decreased ability to repair DNA damage and an increase in apoptosis. [5]
PARP-1, a nuclear enzyme that synthesizes ADP-ribose polymers from NAD, specifically binds Zn2+ and DNA, and recognizes single-strand breaks in DNA. It is involved in base excision repair, both short-patch and long-patch, rejoining DNA strand breaks and plays a role in p53 expression and activation. A high level of basal neuronal DNA damage and PARP activity has been reported in rat brain tissue. PARP-1 was shown to be required for HIV-1 integration into DNA. If PARP-1 is deficient there is no productive HIV-1 infection.
Poly(ADP-ribose) polymerase 1 (PARP1) is essential for diverse functions like DNA damage detection and repair, chromatin modification, cell differentiation, transcription and apoptotic cell death. It also plays a major role in spermatogenesis. Polymorphism in the gene PARP1 is associated with oligospermia and causes male infertility.[2]
Physical form
Supplied as a solution in 20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM DTT, 0.1% Triton™-X 100, 50 % glycerol, and 0.1 mg/ml BSA.
Other Notes
One unit will incorporate 10 femptomole of poly(ADP-ribose) from NAD into 5 μg of immobilized histone proteins in 30 minutes at 22 °C in a 96 well plate.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information human ... PARP1(142) | Gene Information human ... PARP1(142) | Gene Information human ... PARP1(142) | Gene Information human ... PARP1(142) |
| recombinant expressed in E. coli | recombinant expressed in baculovirus infected insect cells | recombinant - | recombinant - |
| form solution | form aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. −20°C | storage temp. −70°C | storage temp. −20°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| shipped in wet ice | shipped in dry ice | shipped in wet ice | shipped in dry ice |
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Danger
hcodes
Hazard Classifications
ED ENV 1
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
9002-93-1
CAS No.
9002-93-1
CAS No.
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