This protein has the sequence of Mouse myeloma cell line, NS0-derived human Osteopontin/OPN protein, Ile17-Asn300, with a C-terminal 6-His tag. The amino acids of Ile17-Asn300 can be found in this article; https://www.ncbi.nlm.nih.gov/protein/NP_000573.1
O4264
Human Osteopontin
recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
Synonym(s):
Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
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| Size/SKU | Availability | Price |
|---|---|---|
50 μg | Please contact Customer Service for Availability | PLN 2,420.00 PLN 2,057.00 |
About This Item
Biological source:
human
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Mol wt:
60-65 kDa by SDS-PAGE (reducing)
Assay:
≥95% (SDS-PAGE)
PLN 2,057.00
List PricePLN 2,420.00Save 15%Please contact Customer Service for Availability
Product Name
Osteopontin human, recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in NSO cells
sterility
sterile
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
60-65 kDa by SDS-PAGE (reducing)
packaging
pkg of 50 μg
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
Gene Information
human ... SPP1(6696)
General description
Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.
Application
Anti-Osteopontin antibody produced in rabbit has been used for ELISA.
Biochem/physiol Actions
A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.
Analysis Note
Bioactivity Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is is 0.100-0.600 ug/mL.
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This Item | |||
|---|---|---|---|
| sterility sterile | sterility - | sterility sterile | sterility sterile |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source human | biological source human | biological source mouse | biological source bovine milk |
| assay ≥95% (SDS-PAGE) | assay ≥97% (HPLC), ≥97% (SDS-PAGE) | assay >95% (SDS-PAGE) | assay >85% (by SDSPAGE under reducing conditions and visualized by silver stain) |
| Quality Level 100 | Quality Level - | Quality Level 100 | Quality Level 100 |
| mol wt 60-65 kDa by SDS-PAGE (reducing) | mol wt 60.0-65.0 kDa | mol wt 30 kDa by SDS-PAGE (reducing), 65 kDa by SDS-PAGE (reducing) (doublet) | mol wt ~60 kDa (reducing conditions) |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the amino acid sequence of Osteopontin human O4264?
What is the amino acid sequence of Osteopontin human O4264?
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