N9914

Polynucleotide phosphorylase from Synechocystis sp.

recombinant, expressed in E. coli

• Synonym(s): PNPase, Polyribonucleotide Nucleotidyltransferase
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 2.7.7.8 (BRENDA, IUBMB)
• MDL number: MFCD00131992
• Specific activity: ≥500 units/mg protein
• Assay: 90% (SDS-PAGE)
• Biological source: bacterial (Synechocystis sp.)
• Recombinant: expressed in E. coli

Properties

• biological source: bacterial (Synechocystis sp.)
• Quality Segment: 200
• recombinant: expressed in E. coli
• description: Histidine tagged
• assay: 90% (SDS-PAGE)
• form: solution
• specific activity: ≥500 units/mg protein
• mol wt: 85 kDa
• technique(s): cell based assay: suitable
• suitability: suitable for molecular biology
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase. [1]

Application

Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. [2] It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor. [3]

Biochem/physiol Actions

Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity.

Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells. [4]

Other Notes

One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C.

Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl₂, 60 mM KCl, 20% (w/v) Glycerol

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable