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N3254

4-Nitrophenyl phosphate di(tris) salt

phosphatase substrate, ≥97% (enzymatic), powder

Synonym(s):

4-Nitrophenyl phosphate bis(tris) salt

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Size/SKUAvailabilityPrice
5 g
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PLN 471.00
1 g
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PLN 707.00
25 g
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PLN 1,650.00
100 g
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PLN 38,980.00

About This Item

Linear Formula:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS Number:
Molecular Weight:
461.36
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
269-198-6
MDL number:
Beilstein/REAXYS Number:
1578142

PLN 471.00


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Product Name

4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

Quality Segment

assay

≥97% (enzymatic)

form

powder

solubility

water: 50 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

Application

4-Nitrophenyl phosphate di(tris) salt has been used:
  • as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates[1]
  • to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)[2]
  • for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)[2]

Biochem/physiol Actions

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

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This Item
73737N3129N3002
assay

≥97% (enzymatic)

assay

≥97.0% (enzymatic)

assay

≥97% anhydrous basis

assay

≥99% (TLC)

solubility

water: 50 mg/mL, clear, colorless to light yellow

solubility

H2O: 0.1 g/mL, clear

solubility

water: 10 mg/mL, clear, colorless to faintly yellow

solubility

water: 20 mg/mL, clear to very slightly hazy

Quality Level

100

Quality Level

100

Quality Level

300

Quality Level

200

form

powder

form

powder or crystals

form

powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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