N2912

Anti-Neurofilament 160/200 antibody, Mouse monoclonal

~2 mg/mL, clone RMdO20, purified from hybridoma cell culture

• Synonym(s): Anti-NEF3
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164708
• Conjugate: unconjugated
• Clone: RMdO20, monoclonal
• Application: ARR, ICC, IHC, WB
• Citations: 48

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: RMdO20, monoclonal
• form: buffered aqueous solution
• species reactivity: mouse, rat, human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, microarray: suitable, western blot: 1-2 μg/mL using extracts of rat brain S1 fraction.
• isotype: IgG1
• UniProt accession no.: P07197
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NEFH(4744), NEFM(4741) ; mouse ... Nefh(380684), Nefm(18040) ; rat ... Nefh(24587), Nefm(24588)

Description

General description

Neurofilaments are 10 nm in diameter and belong to intermediate filament protein family. Neurofilaments are expressed mainly in cells or tissues of neuronal origin and are crucial for proper radial growth of axons. Monoclonal anti-neurofilament 160/200 antibody can be used in ELISA and immunoblotting. It can also be used in microarray. Mouse anti-neurofilament 160/200 antibody reacts specifically with the non-phosphorylated form of NF-M and NF-H (NF-M/H, approx.160 and 200 kDa) in mouse, rat and human.

Immunogen

purified mid-size rat neurofilament (NF-M) subunit.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Monoclonal anti-neurofilament 160/200 antibody can be used in immunocytochemistry (diluted 1: 100) and western blotting (diluted 1: 1,000) . It can also be used in immunohistochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)