M9432

Anti-Macrophage Inflammatory Protein-2, Viral antibody produced in goat

affinity isolated antibody, lyophilized powder

• Synonym(s): Anti-MIP 2, Viral
• UNSPSC Code: 51111800
• MDL number: MFCD02096845
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i), WB
• Citations: 3

Properties

• biological source: goat
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: viral
• technique(s): indirect ELISA: 0.5-1.0 μg/mL, western blot: 0.1-0.2 μg/mL
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

The genome of Kaposi′s sarcoma-associated herpes virus (KSHV) encodes two gene products that are similar to the sequence of human chemokine, MIP-1α. These are named according to the human protein counterpart as viral macrophage inflammatory protein (vMIP)-I and –II. The sequence identity of vMIP-I and vMIP-II to human MIP-1α is 37.9% and 41.1%, respectively. The expression of vMIP-1 and vMIP-2 are reportedly induced by phorbol esters in latently infected lymphoma cells. Both vMIP-I and vMIP-II partially inhibit the infection of peripheral blood mononuclear cells by HIV. vMIP-II also reportedly blocks the infection of CD4-positive cell line expressing CCR3 by HIV, type-1
Anti-Macrophage Inflammatory Protein-II (MIP-II) recognizes recombinant viral MIP-II. The antibody shows less than 10% cross reactivity with recombinant viral MIP-I and recombinant human MIP-1b.

Immunogen

recombinant viral MIP 2, expressed in E. coli.

Application

Anti-Macrophage Inflammatory Protein-II (MIP-II) antibody may be used in immunoblotting at a working concentration of 0.1-0.2 μg/ml. For ELISA the recommended concentration is 0.5-1.0 μg/ml.

Biochem/physiol Actions

The antibody will recognize recombinant viral MIP-2. The antibody shows <10% cross-reactivity with recombinant viral MIP-1 and recombinant human MIP-1β.

Physical form

Lyophilized from a 0.2 μm-filtered solution in phosphate-buffered saline.