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M9004

Malic Dehydrogenase from bovine heart

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

Synonym(s):

L-Malate:NAD+ oxidoreductase, MDH

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Size/SKUAvailabilityPrice
5000 units
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945,00 PLN
10000 units
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1.750,00 PLN
25000 units
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3.790,00 PLN

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-622-5
MDL number:
EC Number:
Specific activity:
2000-4000 units/mg protein (modified Warburg-Christian)
Biological source:
bovine heart

945,00 PLN


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biological source

bovine heart

Quality Segment

form

ammonium sulfate suspension

specific activity

2000-4000 units/mg protein (modified Warburg-Christian)

mol wt

35 kDa

impurities

≤0.01% Glutamic-Oxalacetic Transaminase, ≤0.01% Glutamic-Pyruvic Transaminase

storage temp.

2-8°C

General description

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed. [1]
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Application

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas. [2]

Physical form

Suspension in 3 M (NH4)2SO4 - 0.01 M KH2PO4 solution, pH 7.3

Other Notes

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

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This Item
M1567M2634L2625
biological source

bovine heart

biological source

-

biological source

-

biological source

-

specific activity

2000-4000 units/mg protein (modified Warburg-Christian)

specific activity

≥600 units/mg protein (biuret)

specific activity

600-1000 units/mg protein (biuret)

specific activity

≥500 units/mg protein

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

mol wt

35 kDa

mol wt

-

mol wt

-

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

≤0.01% Glutamic-Oxalacetic Transaminase, ≤0.01% Glutamic-Pyruvic Transaminase

impurities

-

impurities

-

impurities

-


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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