L9545

Leukemia Inhibitory Factor human, animal component free

recombinant, expressed in rice, 500 μg/mL

• Synonym(s): Leukemia Inhibitory Factor human, LIF, rLIF
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00163950

Properties

• recombinant: expressed in rice
• Quality Segment: 200
• assay: >95% (SDS-PAGE)
• potency: >1 x 10⁸ units/mg ED₅₀
• concentration: 500 μg/mL
• technique(s): cell culture | mammalian: suitable
• impurities: <0.05 EU/μg endotoxin
• UniProt accession no.: P15018
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... LIF(3976)

Description

Biochem/physiol Actions

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein that inhibits the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Physical form

0.2 μm filtered solution at 500 μg/mL in buffered saline with 0.02% TWEEN20

Other Notes

rhLeukemia Inhibitory Factor manufactured in the USA by InVitria.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable