L8402

Lysozyme from human neutrophils

≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

• Synonym(s): Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS Number: 9001-63-2
• NACRES: NA.54
• UNSPSC Code: 12352204
• MDL number: MFCD00131557
• EC Number: 3.2.1.17 (BRENDA, IUBMB)
• Specific activity: ≥100,000 units/mg protein (E1%/280)
• Assay: ≥95% (SDS-PAGE)
• Biological source: human neutrophils
• Recombinant: expressed in E. coli

Properties

• biological source: human neutrophils
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• specific activity: ≥100,000 units/mg protein (E1%/280)
• technique(s): cell based assay: suitable
• suitability: suitable for cell lysis
• UniProt accession no.: P61626
• application(s): cell analysis
• storage temp.: −20°C
• SMILES string: N1([C@@H](CCC1)C(=O)N[C@@H](CCC(=O)N)C(=O)NCC(=O)N[C@@H]([C@H](CC)C)C(=O)N[C@@H](CCCN\C(=N\[H])\N)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc4c5c([nH]c4)cccc5)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc2c3c([nH]c2)cccc3)C(=O)N[C@@H](CCCN\C(=N\[H])\N)C(=O)N[C@@H]
• InChI: 1S/C99H159N37O23/c1-11-50(8)77(132-72(139)46-120-81(145)63(32-33-70(101)137)124-88(152)69-31-21-39-136(69)93(157)68(43-73(140)141)131-84(148)62(29-19-37-116-98(109)110)125-90(154)75(48(4)5)135-91(155)76(49(6)7)133-80(144)57(100)24-16-34-113-95(103)104)92(156)126-60(27-17-35-114-96(105)106)82(146)121-51(9)78(142)129-66(41-54-45-119-59-26-15-13-23-56(54)59)87(151)134-74(47(2)3)89(153)122-52(10)79(143)128-65(40-53-44-118-58-25-14-12-22-55(53)58)85(149)123-61(28-18-36-115-97(107)108)83(147)130-67(42-71(102)138)86(150)127-64(94(158)159)30-20-38-117-99(111)112/h12-15,22-23,25-26,44-45,47-52,57,60-69,74-77,118-119H,11,16-21,24,27-43,46,100H2,1-10H3,(H2,101,137)(H2,102,138)(H,120,145)(H,121,146)(H,122,153)(H,123,149)(H,124,152)(H,125,154)(H,126,156)(H,127,150)(H,128,143)(H,129,142)(H,130,147)(H,131,148)(H,132,139)(H,133,144)(H,134,151)(H,135,155)(H,140,141)(H,158,159)(H4,103,104,113)(H4,105,106,114)(H4,107,108,115)(H4,109,110,116)(H4,111,112,117)/t50-,51-,52-,57-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,74-,75-,76-,77-/m0/s1
• InChI key: ZJCXKXFAOCLSRV-RYLVIXIQSA-N
• Gene Information: human ... LYZ(4069)

Description

General description

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

Application

Lysozyme from human neutrophils has been used in the checkerboard method for establishing a synergy between peptidomimetic 1 and immune effector molecules against E. coli.

Biochem/physiol Actions

Lysozymes participates in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

Physical form

Lyophilized from 50 mM sodium acetate, pH 6.0, with 100 mM NaCl

Other Notes

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable