L7261

Lipopolysaccharides from Salmonella enterica serotype typhimurium

purified by trichloroacetic acid extraction

• Synonym(s): LPS
• UNSPSC Code: 12352201
• NACRES: NA.25
• MDL number: MFCD00164404

Properties

• biological source: Salmonella enterica (Serotype typhimurium)
• Quality Segment: 200
• form: lyophilized powder
• purified by: trichloroacetic acid extraction
• impurities: 1-10% Protein (Lowry)
• color: white to faint yellow
• solubility: water: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

This product is TCA extracted from Salmonella typhimurium. The source strain is ATCC 7823. This LPS serotype has been used to induce NOS in rats and guinea pigs.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)