L3759

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

biotin conjugate, lyophilized powder

• Synonym(s): Bandeirea simplicifolia agglutinin, BS-I
• NACRES: NA.32
• UNSPSC Code: 12352200

Properties

• conjugate: biotin conjugate
• form: lyophilized powder
• potency: <250 μg per mL agglutination activity (using human blood group A erythrocytes.)
• composition: Protein, ~85% E1%/280
• extent of labeling: ~5 mol biotin per mol protein
• storage temp.: 2-8°C

Description

Application

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used:

• for highlighting murine endothelial cells
• to identify fetal capillaries in endothelial immunohistochemical staining, conjugated with biotin
• to detect tumor total vessels

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Physical form

Contains sodium citrate

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B₄, BSI-AB₃, BSI-A₂B₂, BSI-A₃B and BSI-A₄. BSI-B₄ is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A₄ has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)