LB - Luria low salt is available in both powder (L3397) and powder with agar formats (L3272).
L3397
LB Broth (Luria low salt)
Powder microbial growth medium
Synonym(s):
Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
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| Size/SKU | Availability | Price |
|---|---|---|
250 g | Please contact Customer Service for Availability | PLN 198.00 |
1 kg | Please contact Customer Service for Availability | PLN 929.00 |
About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
grade
Molecular Biology
Quality Segment
sterility
non-sterile
form
powder
composition
NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(1.55% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
suitable for molecular biology, nonselective for Escherichia coli, nonselective for coliforms
General description
Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.
Application
LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.[1]
Features and Benefits
Luria′s low salt LB powder provides:
- Standard formulation
- A budget-friendly alternative to liquid
- Easy scale-up using larger package sizes
Preparation Note
1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.
2. Autoclave for 15 minutes at 121°C.
Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
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This Item | |||
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable |
| suitability suitable for molecular biology, nonselective for Escherichia coli, nonselective for coliforms | suitability - | suitability - | suitability - |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| composition NaCl, 0.5 g/L , Yeast Extract, 5 g/L , Tryptone, 10 g/L | composition Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages | application(s) food and beverages |
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the difference between the LB - Luria low salt products?
1 answer-
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Helpful?
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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