I4276

Interferon-αA human

≥95%, suitable for cell culture, buffered aqueous solution, recombinant, expressed in E. coli

• Synonym(s): IFN-αA, Interferon-α2A
• CAS Number: 105388-21-4
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD01635115

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥95%
• form: buffered aqueous solution
• mol wt: 19 kDa
• packaging: pkg of 20 μg
• concentration: ≥0.2 mg/mL
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: colorless
• UniProt accession no.: P01563
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IFNA2(3440)

Description

Application

Interferon-αA human has been used to:

• study the effect of Chikungunya virus on interferon-mediated JAK (Janus kinase)-STAT (signal transducers and activators of transcription) signaling[1]
• study the effect of interferon-α and PRK2 (protein kinase C-related kinase 2) inhibitors on hepatitis C virus replication[2]
• compare the effect of Monascus pigment derivatives on hepatitis C virus replication[3]

Biochem/physiol Actions

Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities. Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes) and ruminant embryos (τ subtypes). Interferon induces a variety of effects on target cells including antiviral, antiproliferative and immunomodulatory activities.

Physical form

Supplied as a solution in 5 mM sodium acetate, pH 4.0.

Analysis Note

The biological activity is determined in the cytopathic effect inhibition assay using MDBK cells with vesicular stomatitis virus (VSV). A cytopathic effect of 50% is produced with 1 unit/ml interferon. The units are determined with respect to WHO International standard reference for human interferonαA.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves