I4032

Interleukin-21 from mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-21
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD03703112

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 40-120 ng/mL ED₅₀
• mol wt: predicted mol wt ~14.4 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: Q9ES17
• storage temp.: −20°C
• Gene Information: mouse ... Il21(60505)

Description

Biochem/physiol Actions

IL-21 (interleukin-21) belongs to the IL-2 cytokine family. It is secreted by CD4⁺ T, natural killer (NK) and Th17 cells. IL-21 associates with IL-21 receptor and participates in B-cell proliferation, differentiation as well as apoptosis. It also promotes the cytotoxic activity of CD8⁺ T cells and natural killer cells.IL-21 plays a crucial role in the differentiation of follicular helper T cells. It also exhibits antitumor activity.[1][2] It is also involved in the activation of JAK-STAT (Janus kinase - signal transducer and activator of transcription), PI3K (phosphatidylinositol 3-kinase) and MAPK (mitogen activated protein kinase) pathways.[3]

IL-21 and its receptor have important roles in T and B cell proliferation and natural killer cell maturation.

IL-21 and its receptor have important roles in T and B cell proliferation and natural killer cell maturation. Human and mouse IL-21 share 57% amino acid identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to induce proliferation of human N1186 T cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)