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GE28-9365-40

HiPrep™ SP XL 16/10

Cytiva 28-9365-40

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About This Item

UNSPSC Code:
47101511
NACRES:
NA.56

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pkg of 20 mL

manufacturer/tradename

Cytiva 28-9365-40

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

bed size

16 mm × 100 mm

bed volume

20 mL

column I.D.

16 mm

matrix

6% cross-linked agarose with dextran surface extenders

particle size

45-165 μm

average diameter

90 μm

cleaning

3-14

working range

4-13

suitability

suitable for bioprocess medium

General description

HiPrep™ SP XL 16/10 is a strong cation exchanger prepacked with Q Sepharose™ XL columns for inital capture of proteins and other biomoleculs with ion exchange (IEX) chromatography.

Application

HiPrep™ SP XL 16/10 is a prepacked, ready to use column for ion exchange chromatography. It provides fast, preparative separations of Proteins and other biomolecules.

Features and Benefits

  • Provide high loading capacity and fast purifications.
  • Optimized for reliable, reproducible separations.
  • Compatible with simple pump-based configurations and chromatography systems such as ÄKTA® design.
  • Excellent choice for rapid enrichment during initial capture of Proteins from start material.
  • Strong Sulfopropyl (SP) cation exchanger.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service