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G4423

β-Glucanase from Trichoderma longibrachiatum

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Synonym(s):

endo-1,3(4)-β-glucanase, endoglucanase

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Size/SKUAvailabilityPrice
100 g
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PLN 779.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥1.0 units/mg solid

PLN 779.00


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form

powder

Quality Segment

specific activity

≥1.0 units/mg solid

composition

protein, ≥10%

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
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sustainability

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General description

β -Glucanase represents a group of carbohydrate enzymes which break down glycosidic bonds within beta-glucan.[1] It forms the main constituent of fungal cell walls and could be a potential structural and storage polysaccharide of marine macro-algae. It has the ability to degrade fungal cell walls and may be involved in defense mechanism of plants against pathogenic fungi.[1]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Application

β -Glucanase was used as a cellulase enzyme in the combined biological and chemical pretreatment method for lignocellulosic ethanol production from energy cane. It was also used in the enzymatic saccharification of cellulose and production of ethanol.

Biochem/physiol Actions

A mixture composed mainly of β-1→3 / 1→4-glucanase, xylanase, and cellulase activities. β-glucosidase, β-xylosidase, α-L-arabinofuranosidase, amylase, and protease activities are also present.[2][3]

Physical form

contains maltodextrin, silica and sodium benzoate

Other Notes

One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).

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This Item
G0251X3876C6137
specific activity

≥1.0 units/mg solid

specific activity

≥1,000,000 units/g solid

specific activity

≥100 units/mg protein

specific activity

≥200 units/g solid

form

powder

form

solid

form

lyophilized powder

form

lyophilized powder (essentially salt free)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

composition

protein, ≥10%

composition

-

composition

-

composition

-

sustainability

Greener Alternative Product

sustainability

-

sustainability

Greener Alternative Product

sustainability

-


Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Certificates of Analysis (COA)

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Questions

1–3 of 3 Questions  
  1. How much protease activity is present?

    1 answer
    1. The minimum activity of this ?-glucanase is 1 unit per milligram. The activity varies from lot to lot and is reported in the Certificate of Analysis. This product has not been tested for any additional protease activity. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/g4423#product-documentation

      Helpful?

  2. Is this product suitable for use in food preparation?

    1 answer
    1. No. Unless otherwise stated, this product is intended for research use only. It is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals.

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  3. Hi, I am planning to use β-Glucanase G4423 to digest the Pyhtium inosidieus cell wall to let the probes for In situ hybridozation . May I know the conditions, concentration and dilution buffer for this product? Thanks Best

    1 answer
    1. This product has not been testd in In Situ Hybridization in house. Below is a link to an article citing this product in ISH.

      https://www.nature.com/articles/s42003-021-02693-y

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