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FD40S

Fluorescein isothiocyanate–dextran

average mol wt 40,000

Synonym(s):

FITC–Dextran

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100 MG

PLN 501.00

250 MG

PLN 802.00

1 G

PLN 2,540.00

PLN 501.00

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About This Item

CAS Number:
60842-46-8
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
MFCD00131092

Key Documents

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biological source

synthetic (organic)

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 40,000

extent of labeling

0.003-0.020 mol FITC per mol glucose

color

yellow to dark orange

solubility

H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange

storage temp.

2-8°C

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1 of 4

This Item
FD40FD20FD20S
Fluorescein isothiocyanate–dextran average mol wt 40,000

FD40S

Fluorescein isothiocyanate–dextran

Fluorescein isothiocyanate–dextran average mol wt 40,000

Sigma-Aldrich

FD40

Fluorescein isothiocyanate–dextran

Fluorescein isothiocyanate–dextran average mol wt 20,000

Sigma-Aldrich

FD20

Fluorescein isothiocyanate–dextran

Fluorescein isothiocyanate–dextran average mol wt 20,000

Sigma-Aldrich

FD20S

Fluorescein isothiocyanate–dextran

biological source

synthetic (organic)

biological source

bacterial (Leuconostoc mesenteroides)

biological source

bacterial (Leuconostoc mesenteroides)

biological source

sugar beets root

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

solubility

H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange

solubility

H2O: 25 mg/mL

solubility

water: 25 mg/mL

solubility

water: 25 mg/mL

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

yellow to dark orange

color

light yellow to dark orange

color

light yellow to dark orange

color

yellow to dark yellow

General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% α-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. FITC-dextran has an excitation maximum of 490 nm. It has a pH range of 5 to 9. [1]

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
Fluorescein isothiocyanate–dextran (FITC-dextran, fluorescein-dextran, FITC) was used in the following processes:
  • Fluorescein-dextran microscopy.[2][3][4]
  • To evaluate the in vivo pulmonary vascular permeability in polymorphonuclear leukocyte α-defensins expressing transgenic mice.[5]
  • To quantify endocytic activity by flow cytometry.[6]
  • To label α-lactalbumin and ribonuclease.[7]
FITC-dextran is suitable for extensive microcirculation and cell permeability research utilizing microfluorimetry. It is also suitable for the study of plant cell wall porosity and capillary permeability.

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
Similar to FD-40, but produced by Sigma.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000542242
0000542240
0000542241
0000538995
0000515997

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Kristina Krempaska et al.
Respiratory research, 21(1), 25-25 (2020-01-17)
Idiopathic pulmonary fibrosis (IPF) is a chronic fatal lung disease without a cure and new drug strategies are urgently needed. Differences in behavior between diseased and healthy cells are well known and drug response can be different between cells isolated
Molecular weight, molecular-weight distribution and molecular size of native dextran.
AROND L H and H P FRANK
The Journal of Physical Chemistry, 953 - 957 (1954)
Erika M J Siren et al.
Scientific reports, 8(1), 7581-7581 (2018-05-17)
Here we present a simple technique for re-directing reactions on the cell surface to the outermost region of the glycocalyx. Macromolecular crowding with inert polymers was utilized to reversibly alter the accessibility of glycocalyx proteoglycans toward cell-surface reactive probes allowing
Yun Mi Lee et al.
Journal of diabetes research, 2013, 245271-245271 (2013-12-29)
Retinal pathogenic angiogenesis in the eyes is a causative factor in retinopathy of prematurity, diabetic retinopathy, and age-related macular degeneration. This study was designed to examine the pathogenic role of the high-mobility group box-1 (HMGB1) protein and the inhibitory effect
The structure of NRRL B-512F dextran, methylation studies.
Van Cleve JW, Schacfer WC and Rist CE
Journal of the American Chemical Society, 4435 - 4438 null
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Related Content

Izotiocyjanian fluoresceiny-Dekstran

Dekstran jest polimerem anhydroglukozy. Składa się w około 95% z wiązań alfa-D-(166). Pozostałe (163) wiązania odpowiadają za rozgałęzienie dekstranu.

Fluorescein Isothiocyanate-Dextran

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

Product Information - FD40S

Global Trade Item Number

SKUGTIN
FD40S-250MG04061833622520
FD40S-100MG04061833622513
FD40S-1G04061826754573

Questions

1–3 of 3 Questions  

  1. Can I dissolve this FITC-dextran in PBS to a concentration of 10 mg/mL as a stock solution, and then aliquot into small tubes kept in a box sheltered from light at -40 degrees Celcius?

    1 answer

    1. This product is routinely tested for solubility at 25 mg/mL in water. Solubility or solution stability in PBS has not been tested. Solubility in PBS is expected, as it is a common buffer used in various protocols. The cited protocol recommends storage on ice or at 4 °C wrapped in aluminum foil. No specific time frame for storage is provided, and no information regarding storage at -40 °C exists.

      Koester ST, Li N, Lachance DM, Dey N. Marker-based assays for studying gut transit in gnotobiotic and conventional mouse models. STAR Protoc. 2021 Nov 5;2(4):100938. doi: 10.1016/j.xpro.2021.100938. PMID: 34806048; PMCID: PMC8585659.

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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