F4771

Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse

clone 1B10, ascites fluid

• Synonym(s): Fibroblast Surface Protein Antibody, Fibroblast Surface Protein Antibody - Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164646
• Conjugate: unconjugated
• Clone: 1B10, monoclonal
• Application: FACS, IHC (f), complement-mediated cytotoxicity assay
• Citations: 25

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 1B10, monoclonal
• mol wt: antigen 43-72-80 kDa
• contains: 15 mM sodium azide
• species reactivity: human
• technique(s): complement-mediated cytotoxicity assay: 1:500, flow cytometry: suitable, immunohistochemistry (frozen sections): suitable
• isotype: IgM
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Human Fibroblast Surface Protein (mouse IgM isotype) is derived from the 1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cultured human thymic fibroblasts. Human fibroblast surface protein is a fibroblast antigen that is expressed mainly on human synovial, foreskin and thymic fibroblasts and is absent on human epithelial cells and lymphocytes.

This fibroblast antigen has been demonstrated on human synovial, mammary, foreskin and thymic fibroblasts and in malignant fibrosarcoma tissue. The antigen is both cell membrane and lysosome associated.

Immunogen

cultured human thymic fibroblasts

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse has been used in:

• immunofluorescence
• flow cytometry
• immunohistochemistry
• immunoblotting

Monoclonal anti-fibroblast surface protein antibody can be used in flow cytometry of human fibroblasts and monocytes. It is also useful in immunoblotting and western blotting.

Biochem/physiol Actions

Monoclonal Anti-Fibroblast Surface Protein antibody is also used to remove fibroblasts from human cultured cells by inhibiting fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells.

Analysis Note

Working dilution is at least 1:500 by complement-mediated microcytotoxicity assay.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable