E2513
ExtrAvidin®-Agarose, high binding
For purification of biotinylated macromolecules and complexes
Synonym(s):
ExtrAvidin® beads, de-glycosylated Avidin
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Please contact Customer Service for Availability | PLN 862.00 |
5 mL | Please contact Customer Service for Availability | PLN 2,850.00 PLN 2,422.50 |
About This Item
NACRES:
NA.56
UNSPSC Code:
41106500
Form:
aqueous suspension, suspension
PLN 862.00
Please contact Customer Service for Availability
form
aqueous suspension, suspension
feature
For purification of biotinylated macromolecules and complexes
concentration
50%
matrix
4% agarose (beaded)
matrix activation
cyanogen bromide
storage temp.
2-8°C
General description
ExtrAvidin® is a unique de-glycosylated Avidin modified form of an affinity purified egg white avidin. The ExtrAvidin is conjugated to cyanogen bromide-activated agarose beads.
ExtrAvidin® is a tetrameric protein containing four biotin binding sites. The Avidin-Biotin high affinity interaction (Kd=10-15M) is considered one of the strongest non-covalent interactions known in nature. The use of Avidin-Biotin complex for affinity purification was described in the first half of 1970 and since then it was successfully utilized in numerous studies and biotechnological applications.1-3 This was achieved due to the ability to chemically coupe the small Biotin molecule with different binders, without disturbing its function or structure, thus allowing unique interaction with a variety of Avidin carriers including: protein or DNA molecules, Avidin protein bound to a solid surface matrix, reporter molecules, probes or carriers,. Among the known Avidin-Biotin interaction based applications are purification, enrichment, detection, amplification and other research medical and industrial processes. In addition, the ExtrAvidin® high specificity binding to Biotin, together with the low background staining, grant it significant advantage compared to the non-modified Avidin or Streptavidin produced by Streptomyces avidinii.
ExtrAvidin® is a tetrameric protein containing four biotin binding sites. The Avidin-Biotin high affinity interaction (Kd=10-15M) is considered one of the strongest non-covalent interactions known in nature. The use of Avidin-Biotin complex for affinity purification was described in the first half of 1970 and since then it was successfully utilized in numerous studies and biotechnological applications.1-3 This was achieved due to the ability to chemically coupe the small Biotin molecule with different binders, without disturbing its function or structure, thus allowing unique interaction with a variety of Avidin carriers including: protein or DNA molecules, Avidin protein bound to a solid surface matrix, reporter molecules, probes or carriers,. Among the known Avidin-Biotin interaction based applications are purification, enrichment, detection, amplification and other research medical and industrial processes. In addition, the ExtrAvidin® high specificity binding to Biotin, together with the low background staining, grant it significant advantage compared to the non-modified Avidin or Streptavidin produced by Streptomyces avidinii.
Application
ExtrAvidin®-Agarose provides high affinity with high specificity binding to Biotin (also known as vitamin B7) including biotinylated proteins and biotin-tagged fusion proteins. The product may be used in various immunological techniques, including Immunoprecipitation and Immunoaffinity purification.
Physical form
ExtrAvidin®-Agarose is provided as suspension at a 1:1 ratio, in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
DO NOT FREEZE
This product is for research use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
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This Item | |||
|---|---|---|---|
| concentration 50% | concentration - | concentration - | concentration - |
| form aqueous suspension | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| matrix 4% agarose (beaded) | matrix - | matrix - | matrix - |
| feature For purification of biotinylated macromolecules and complexes | feature - | feature - | feature - |
| matrix activation cyanogen bromide | matrix activation - | matrix activation - | matrix activation - |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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