D2192
Desthiobiotin polyethyleneoxide Iodoacetamide
≥90% (HPLC)
Synonym(s):
Desthiobiotin PEO iodoacetamide
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About This Item
Empirical Formula (Hill Notation):
C21H38IN5O6
Molecular Weight:
583.46
UNSPSC Code:
12352200
PubChem Substance ID:
MDL number:
assay
≥90% (HPLC)
form
powder
solubility
H2O: 10 mg/mL
storage temp.
2-8°C
SMILES string
CC1NC(=O)NC1CCCCCC(=O)NCCC(=O)NCCOCCOCCNC(=O)CI
InChI
1S/C21H38IN5O6/c1-16-17(27-21(31)26-16)5-3-2-4-6-18(28)23-8-7-19(29)24-9-11-32-13-14-33-12-10-25-20(30)15-22/h16-17H,2-15H2,1H3,(H,23,28)(H,24,29)(H,25,30)(H2,26,27,31)
InChI key
DBOWCCSTSCXTEL-UHFFFAOYSA-N
Application
Especially useful for cysteine labeling in many proteomics applications such as peptide mapping and mass spectrometry.
Features and Benefits
- Easily eluted from streptavidin or avidin affinity matrices under mild conditions
- Incorportates a 16 atom hydrophilic spacer
- Typically coupled to sulfhydryl groups at pH 7.5 - 8.5
Other Notes
Sulfhydryl specific, water soluble biotinylation reagent.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Related Content
Instructions
T P Conrads et al.
Analytical chemistry, 73(9), 2132-2139 (2001-05-17)
We describe the combined use of 15N-metabolic labeling and a cysteine-reactive biotin affinity tag to isolate and quantitate cysteine-containing polypeptides (Cys-polypeptides) from Deinococcus radiodurans as well as from mouse B16 melanoma cells. D. radiodurans were cultured in both natural isotopic
Advances in proteome analysis by mass spectrometry.
T J Griffin et al.
The Journal of biological chemistry, 276(49), 45497-45500 (2001-10-05)
Michael B Goshe et al.
Journal of proteome research, 2(2), 153-161 (2003-04-29)
The ability to identify and quantitate integral membrane proteins is an analytical challenge for mass spectrometry-based proteomics. The use of surfactants to solubilize and facilitate derivatization of these proteins can suppress peptide ionization and interfere with chromatographic separations during microcapillary