AFRC 84B4
Reactivity: Anti-Haemagglutinin, influenza, 94120829
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About This Item
UNSPSC Code:
41106514
biological source
Horse x Mouse/Horse Hybridoma
ImmunoDon
Horse sub-mandibular lymph node
Myeloma
AFRC 17D1
Reactivity
Anti-Haemagglutinin, influenza
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Horse sub-mandibular lymph node x AFRC 17D1
Cell Line Description
The heterohybridoma AFRC 84B4 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 84B4 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).
Immunogen
Influenza A equine 2 virus
Application
Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
Other Notes
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Certificates of Analysis (COA)
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