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product name
A6, 89072613
biological source
toad kidney
growth mode
Adherent
karyotype
Hyperdiploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
South African clawed toad kidney
Cell Line Description
Derived from normal kidneys of an adult male toad (Xenopus laevis). A6 cells support the replication of Granoff′s frog viruses but not the Lucke inclusion-tumour associated virus.
Application
Replication studies
Culture Medium
NCTC 109 + 15% Distilled H2O + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 27-28°C.
Other Notes
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Certificates of Analysis (COA)
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