C7482

Caspase 6 human

recombinant, expressed in E. coli, >90% (SDS-PAGE), lyophilized powder, 13,000 units/mg protein

• Synonym(s): Mch2
• NACRES: NA.32
• UNSPSC Code: 12352200
• Specific activity: 13,000 units/mg protein
• Assay: >90% (SDS-PAGE)
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 100
• assay: >90% (SDS-PAGE)
• form: lyophilized powder
• specific activity: 13,000 units/mg protein
• mol wt: ~30 kDa
• solubility: PBS: soluble
• shipped in: dry ice
• storage temp.: −70°C

Description

Biochem/physiol Actions

Cleaves the substrates consisting of consensus sequence VEID.

Spontaneously undergoes autoprocessing to yield the subunits associated to form the active enzyme.

Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates, or as a positive control in caspase activity assays.

Physical form

Contains 0.052% ammonium sulfate, 0.158% Tris−HCl, 0.76% sodium chloride.

Other Notes

One unit will hydrolyze 1 nmol of the caspase substrate VEID-pNA to VEID and p-nitroaniline per hour at pH 7.2 at 37 °C.