C7104

Anti-Cdc27 antibody,Mouse monoclonal

clone AF3.1, purified from hybridoma cell culture

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01633250
• Conjugate: unconjugated
• Clone: AF3.1, monoclonal
• Application: ARR, ELISA (i), ICC, IHC (p), IP, WB
• Citations: 15

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: AF3.1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 97 kDa
• species reactivity: mouse, Xenopus, rat, bovine, canine, human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using a HeLa Cell nuclear extract
• isotype: IgG2b
• UniProt accession no.: P30260
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CDC27(996) ; mouse ... Cdc27(217232) ; rat ... Cdc27(360643)

Description

General description

Monoclonal Anti-Cdc27 (mouse IgG2b isotype) is derived from the AF3.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminal of human Cdc27, conjugated to KLH.

The gene CDC27 (Cell division cycle protein 27 homolog) is mapped to human chromosome 17q12–23.2. The protein localizes in the nucleus.

Immunogen

synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.

Application

Monoclonal Anti-Cdc27 antibody produced in mouse is suitable for the following applications:

• Immunocytochemistry using 4% paraformaldehyde fixation and Triton X-100 permeabilization
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
• Immunoprecipitation.
• Indirect ELISA
• Microarray
• Western blotting / immunoblotting at a concentration of 1-2μg/mL using a HeLa Cell nuclear extract.
• Anaphase-promoting complex/cyclosome APC/C Purification.

Biochem/physiol Actions

CDC27 (Cell division cycle protein 27 homolog) is a component of anaphase-promoting complex/cyclosome (APC/C), an E3 ligase involved in cell cycle control. Phosphorylation of CDC27 is crucial for tumor growth factorβ (TGFβ)-induced activation of APC. It interacts with Microcephalin (MCPH1), causative gene for primary recessive autosomal microcephaly. DNA-damage response mediator MDC1 interacts with APC/C through CDC27. Down-regulation of CDC27 in irradiated cervical cancer cell line results in greater survival fraction. Polymorphism in CDC27 are linked with breast cancer.

The Cdc16/27/34 complex or the Anaphase-promoting complex/cyclosome (APC/C) complex functions as an ubiquitin conjugating enzyme, ubiquitinating cyclin B, and resulting in cyclin B/Cdk complex degradation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.