C5723

Anti-Cytochrome c antibody produced in sheep

whole antiserum

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01864291
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, IP, WB
• Citations: 6

Properties

• biological source: sheep
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen 14.4 kDa
• species reactivity: human, rabbit, canine, rat
• technique(s): immunoprecipitation (IP): suitable, indirect immunofluorescence: suitable, western blot: 1:5,000 using MCF-7, Rat-1, MDCK or Jurkat cell extracts
• UniProt accession no.: P99999
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CYCS(54205) ; rat ... Cycs(25309)

Description

General description

Cytochrome c is an electron transport protein released from mitochondria as an early committed event in apoptosis. Cytochrome c and dATP are cofactors for the mammalian apoptosome, which is composed of Apaf-1, Bcl-2, and procaspase 9. When caspase 9 is activated, activation of other caspases follow including the death protease caspase 3. The release of cytochrome c is inhibited by the presence of Bcl-2 on these organelles preventing the initiation of apoptosis. In cells induced by several apoptotic agents (such as UV irradiation, staurosporine, and overexpression of Bax), caspase inhibitors do not prevent cytochrome c release.

Immunogen

rabbit cytochrome c

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable