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C3777

Casein fluorescein isothiocyanate from bovine milk

Type II, essentially salt-free, lyophilized powder

Synonym(s):

FITC-casein

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Size/SKUAvailabilityPrice
25 mg
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PLN 812.00
100 mg
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PLN 2,460.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:

PLN 812.00


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type

Type II

Quality Segment

form

essentially salt-free, lyophilized powder

extent of labeling

20-50 μg FITC per mg solid

solubility

water: 5 mg/mL, clear to hazy, yellow to orange

storage temp.

2-8°C

General description

Casein is a major milk phospho-protein and constitutes about 80% of the total protein content in milk. Bovine milk is composed of four types of casein such as, αS1, αS2, β, and κ. Casein exists as micelles in milk.

Application

highly sensitive protease substrate
Casein fluorescein isothiocyanate from bovine milk has been used:
  • as a substrate for semi-quantitative analysis of protease in Arabidopsis cells
  • to determine the caseinolytic activity of secreted LasB (elastase), alkaline protease and protease IV
  • for determining the SpeB (cysteine protease) proteolytic activity in Streptococcus pyogenes cells

Biochem/physiol Actions

Casein micelle system is involved in the prevention of pathological calcification of mammary glands.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

  1. What is the molecular weight?

    1 answer
    1. Casein is a combination of alpha, beta, and kappa sub-units. These sub-units have a molecular with range of 19 - 25 kDa. The molecular weight of the FITC is 389.382.

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