C-12640

Human Bronchial Epithelial Cells (HBEpC)

500,000 cryopreserved cells

• Synonym(s): HBEpC cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human bronchi
• Morphology: (epithelial)

Properties

• biological source: human bronchi
• product line: PromoCell^®
• packaging: pkg of 500,000 cells
• morphology: (epithelial)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Our HBEpC are now also available from Air-Liquid Interface pre-screened, HLA-typed, and COPD/Asthma donors.

Application

Primary Human Bronchial Epithelial Cells (HBEpC) are isolated from the surface epithelium of human bronchi and are provided in a cryopreserved format. HBEpC stain positive for cytokeratin. The respiratory epithelia are responsible for the lubrication of the lungs, the maintenance of humidity, and the cleaning of the respiratory tract. They are an important target for drugs, toxins, and carcinogens. In addition, they are involved in many diseases such as cystic fibrosis. Consequently, HBEpC are useful for investigating the function and pathology of the respiratory system.

Biochem/physiol Actions

Bronchi

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 10000 - 15000 cells per cm²Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable