C0794
Chitosanase from Streptomyces sp.
buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)
Synonym(s):
Chitosan N-acetylglucosaminohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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form
buffered aqueous glycerol solution
specific activity
≥15 units/mg protein (E1%)
concentration
≥0.5 mg/mL
solubility
0.1 M acetate buffer, pH 5.5: soluble
storage temp.
−20°C
Application
Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.
Biochem/physiol Actions
Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.
Unit Definition
One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.
Physical form
Solution in 50% glycerol containing 50 mM sodium acetate
Preparation Note
Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Boucher, I., et al.
The Journal of Biological Chemistry, 270, 31-31 (1995)
T Fukamizo et al.
The Biochemical journal, 311 ( Pt 2), 377-383 (1995-10-15)
Chitosanase was produced by the strain of Streptomyces lividans TK24 bearing the csn gene from Streptomyces sp. N174, and purified by S-Sepharose and Bio-Gel A column chromatography. Partially (25-35%) N-acetylated chitosan was digested by the purified chitosanase, and structures of
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Illumination with white linearly polarized light (WLPL) stimulated chitinase and chitosanase in their degradation of chitin and chitosan, respectively. Enzymes were illuminated at room temperature in separate vessels, then admixed in reactors containing polysaccharides. Hydrolysis of chitosan to glucosamine followed
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