B1522

Basal Medium Eagle

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): BME
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Product Name: Basal Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• Quality Segment: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: L-glutamine: no; NaHCO₃: yes; Earle’s salts (5% CO₂): yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Eagle′s Basal Medium (BME) is one of the most used synthetic cell culture media. The tissue culture association recommends that the term "Eagle′s Basal Medium" be used only to refer to the formula developed to support HeLa cells. BME has previously been used in monolayer culture studies to assess the growth response of normal (WI-38) and transformed (mouse and HeLa) cells. BME is the precursor of Dulbecco′s Modified Eagle′s medium (DME) and Eagle′s Minimum Essential Medium (MEM).

Application

Basal Medium Eagle has been used:

• as a component in the culture medium for cochlear organotypic cultures and salicylate treatment[1]
• to maintain cerebellar granule neurons (CGNs) to determine if there is a system that regulates the distribution and/or group dynamics of axonal mitochondria[2]
• in the preparation of cortical slices, serum-free medium, and slice culture medium for slice overlay assay to study the influence of extracellular signals on neuronal development[3]

Preparation Note

Supplement with 0.292 g/L L-glutamine.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable