AB0100

Alkaline Phosphatase Blue Microwell Substrate

alkaline phosphatase substrate, solution

• Synonym(s): 5-bromo-4-chloro-3-indolyl phosphate analog / NBT substrate for microwell assays
• UNSPSC Code: 12352204
• NACRES: NA.83

Properties

• Product Name: Alkaline Phosphatase Blue Microwell Substrate, sufficient for 400 mL working substrate
• form: solution
• Quality Segment: 200
• usage: sufficient for 400 mL working substrate
• storage temp.: 2-8°C

Description

General description

Alkaline phosphatase blue microwell substrate, a two-component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require an insoluble reaction product.

Application

Prior to reaction with alkaline phosphatase, the BCIP^® reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.

Physical form

A 2 component kit sufficient for preparing 400 mL of working substrate.

Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids