A8781

β-Amylase from sweet potato

• Synonym(s): 1,4-α-D-Glucan maltohydrolase
• CAS Number: 9000-91-3
• UNSPSC Code: 41106514
• NACRES: NA.56
• EC Number: 232-566-1
• MDL number: MFCD00081391
• EC Number: 3.2.1.2 (BRENDA, IUBMB)

Properties

• form: powder
• Quality Segment: 100
• mol wt: ~200,000
• packaging: vial of 15 mg
• greener alternative product score: old score: 57; new score: 1; Find out more about DOZN™ Scoring
• greener alternative product characteristics: Waste Prevention; Safer Solvents and Auxiliaries; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• greener alternative category: Re-engineered,
• storage temp.: −20°C

Description

General description

β-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (α/β) core domain and a C-terminal loop region.[1] It belongs to the glycoside hydrolase family GH14.[2]

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Application

β-Amylase from sweet potato has been used:

• as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)[3]
• as a standard for calibration curve preparation in size exclusion chromatography
• as a model protein for small-angle X-ray analysis (SAXs)[4]

Biochem/physiol Actions

β-Amylase acts on the polysaccharide α(1,4) glycosidic linkages and releases maltose present in the non-reducing ends.[2] Due to its stability, β-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves