A2856

Anti-ATG7 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): ATG7 Antibody - Anti-ATG7 antibody produced in rabbit, Atg7 Antibody, Anti-APG7, Anti-APG7-like, Anti-APG7L, Anti-Apoptosis-specific-protein, Anti-Autophagy protein 7-like, Anti-Ubiquitin activating enzyme E1-like protein
• MDL number: MFCD09753848
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~75 kDa
• species reactivity: rat, human, mouse
• packaging: antibody small pack of 25 μL
• technique(s): immunoprecipitation (IP): 1-2 μL using human U87 cell lysate; western blot: 0.5-1 μg/mL using whole extracts of mouse 3T3 cells
• UniProt accession no.: O95352
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ATG7(10533) ; mouse ... Atg7(74244) ; rat ... Atg7(312647)

Description

General description

Autophagy is a catabolic process by which cell delivers cytoplasmic constituent into lysosome for protein degradation. 16 ATG genes are essential for autophagosome preparation out of which Atg7 is the one required to activate ubiquitin-like proteins Atg12 and Atg8. Atg7 is also crucial for amino acid supply in neonates, as well as for bulk degradation of proteins and organelles in mice during starvation. Anti-ATG7 antibody can be used in immunohistochemistry and immunoprecipitation. Rabbit anti-ATG7 antibody reacts specifically with Atg7 of human, mouse and rat.

Immunogen

synthetic peptide corresponding to amino acids 38-50 of human ATG7, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse and rat.

Application

Anti-ATG7 antibody can be used in western blotting and immunoblotting.

Anti-ATG7 antibody produced in rabbit has been used:

• for quantitative real-time polymerase chain reaction of autophagy-related genes
• in immunofluorescence and immunoblotting
• in in vivo acetylation/deacetylation of autophagy proteins

Rabbit anti-ATG7 antibody has been used for immunoblotting assays. The antibody is also suitable for use in immunoprecipitation applications at 1-2μL using human U87 cell lysates.

Rabbit anti-ATG7 antibody was used for immunohistochemistry on sections of mouse midbrain fixed in 4% paraformaldehyde at 1:2000.

Biochem/physiol Actions

Autophagy related 7 (ATG7) regulates caspase mediated autophagy and apoptosis. This has a significant effect on tumor progression and ATG7 acts as a tumor suppressor. ATG7 is known to be associated with many cancer types, hence it is considered as a target for cancer therapy. Loss of ATG7 gene contributes to liver carcinogenesis. The ATG7 gene encodes E1-like enzyme essential for lipidation and conjugation systems : ubiquitin-like microtubule-associated protein 1A/1B-light chain 3 (LC3) and autophagy related 12 (ATG12) respectively.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves